This lecture on RNA polymerase structure explains the sigma factor and clefts in RNA polymerase and the role of sigma factor in transcription.
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RNAP is a large molecule. The core enzyme has five subunits (~400 kDa):[5] β': The β' subunit is the largest subunit. The β' subunit contains part of the active center responsible for RNA synthesis and contains some of the determinants for non-sequence-specific interactions with DNA and nascent RNA. β: The β subunit is the second-largest subunit. The β subunit contains the rest of the active center responsible for RNA synthesis and contains the rest of the determinants for non-sequence-specific interactions with DNA and nascent RNA. αI and αII: The α subunit is the third-largest subunit and is present in two copies per molecule of RNAP, αI and αII. Each α subunit contains two domains: αNTD (N-Terminal domain) and αCTD (C-terminal domain). αNTD contains determinants for assembly of RNAP. αCTD (C-terminal domain) contains determinants for interaction with promoter DNA, making non-sequence-non-specific interactions at most promoters and sequence-specific interactions at upstream-element-containing promoters, and contains determinants for interactions with regulatory factors. ω: The ω subunit is the smallest subunit. The ω subunit facilitates assembly of RNAP and stabilizes assembled RNAP.
In order to bind promoters, RNAP core associates with the transcription initiation factor sigma (σ) to form RNA polymerase holoenzyme. Sigma reduces the affinity of RNAP for nonspecific DNA while increasing specificity for promoters, allowing transcription to initiate at correct sites. The complete holoenzyme therefore has 6 subunits: β'βαI and αIIωσ (~450 kDa). Source of the article published in description is Wikipedia. I am sharing their material. © by original content developers of Wikipedia.
Link- http://en.wikipedia.org/wiki/Main_Page Material source: Molecular Biology of the Gene (4th Edition)
James D. Watson (Author), Alan M. Weiner (Author), Nancy H. Hopkins (Author)
Link: http://www.amazon.com/Molecular-Biolo...
RNA polymerase Sigma factor and clefts transcription and translation | |
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